The General Directorate of Food Management (GGALI in Portuguese) under the National Health Surveillance Agency (ANVISA in Portuguese) has published a manual “Sanitary Regulation of Food Supplements.” This material is part of the training itinerary on the Sanitary Regulation of Food Supplements and systematically and progressively compiles the content developed throughout the six modules of the course. The material was designed to facilitate technical understanding and practical application of the current regulatory framework, considering the responsibilities of the regulated sector and the National Health Surveillance System. It addresses the fundamentals that define what dietary supplements are and who they are intended for, the registration and electronic notification procedures, the composition criteria, and the evaluation of the safety and efficacy of their components, as well as the applicable regulations for general and nutritional labeling. The content articulates normative concepts, regulatory flows, and practical examples, allowing the reader to understand not only what the legislation requires but also why these requirements exist and how they should be implemented in daily professional practice.